N. limicola III

 
 
 

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Lab Services:

Filamentous Identification Lab Service. One reason to identify filaments is to determine the filaments characteristics and then determine the type present.  If the type is found out, a root cause can usually be associated with a particular filament.  If the cause is known, then a correction can be made to alleviate problems. Chlorination is only a quick fix.  Without process changes, filaments will grow back after chlorination.

Wastewater Biomass Analyses and Cooling Tower Analyses also available

 

Training Materials:

Training is an integral part of any job. Not everyone is at the same level of training. Many people want beginning concepts and basics. Some need technical information or troubleshooting. Some want equipment, technology or process information.

We have developed a full set of Basic training, Advanced training, Filamentous Identification the Easy Way as well as custom training CD's Manuals. We also provide hands-on training classes and soon will have an Online "E-University".

 


 

                                                                   

 N. limicola III or Isosphaera

 

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We hope you like the new look of our Filamentous Bacteria Identification Sheets

If you would like more information on filaments, you might want to consider purchasing our Filamentous Identification the Easy Way Training materials.

We also have our lab that can perform a Filamentous identification lab analysesof your own MLSS for more information

 

 

Nostocoida limicola III or Isosphaera

What really is this called? We have heard both terms depending upon who's text book you look at. With all the DNA probes, FISH analyses, it becomes confusing as to what is the name of each filament.

 

When trying to determine species, stick to the basics, and focus on the causes and controls of the filaments present. The main point of any filamentous identification is not to get a PhD, but to fix your plant!

 

Identification

Relatively long non-motile filaments (200-300 µm). Bent and irregularly coiled filaments with incidental true branching. Cell septa are clear with indentations. Cells are large and spherical, or oval shaped (1.6-2.0 µm). Filaments are found within the floc structure. The filament staining is variable, although it is usually Gram positive and Neisser positive. Usually easy to identify due to its Neisser staining properties. Stains entirely purple and looks like poppet beads. In bright field or phase contrast, looks like a string of pearls. There is no sheath and there are no sulfur granules. Poly-hydroxybutric acid (PHB) granules are frequently observed as dark intracellular granules. Attached growth is usually uncommon.

    

Similar Organisms:

Three subtypes are known.  Similar to N limicola I also.

 

Environment

This filament is usually found in environments where there is low DO or septicity. Often found with low nutrients and the presence of organic wastes. Wastes containing starch seem more selective to this filament. Bulking is more common in industrial wastes. The filament appears to be facultative fermentative, which is unique for most filaments.  We see this quite often in Chemical, Refineries and Steel mill facilities.

 

Control:

Check solids handling, check DO. We have never seen this filament as a dominant species. Usually is found in low to common levels.

 

For more information on Filamentous Identification

 N. limicola II

 N. limicola I

More photos to come. . .

 

If you need more information on our Filamentous Identification the Easy Way Training CD or on Internet training on Filamentous bacteria, causes and controls.

How and why on Wastewater Biomass Analyses

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