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Filamentous Identification Lab Service. One reason to identify filaments is to determine the filaments characteristics and then determine the type present. If the type is found out, a root cause can usually be associated with a particular filament. If the cause is known, then a correction can be made to alleviate problems. Chlorination is only a quick fix. Without process changes, filaments will grow back after chlorination.
Wastewater Biomass Analyses and Cooling Tower Analyses also available
Training is an integral part of any job. Not everyone is at the same level of training. Many people want beginning concepts and basics. Some need technical information or troubleshooting. Some want equipment, technology or process information.
We have developed a full set of Basic training, Advanced training, Filamentous Identification the Easy Way as well as custom training CD's Manuals. We also provide hands-on training classes and soon will have an Online "E-University".
We hope you like the new look of our Filamentous Bacteria Identification Pages
If you would like more information on filaments, you might want to consider purchasing our Filamentous Identification the Easy Way Training materials.
We also have our lab that can perform a Filamentous identification lab analyses of your own MLSS for more information
This filament is very common in septic environments. Don't get overly technical. When trying to determine species, stick to the basics, and focus on the causes and controls of the filaments present. The main point of any filamentous identification is not to get a PhD, but to fix your plant!
Relatively small, non-motile filaments (50-200 µm). Straight or smoothly curved filaments with no branching. Cells are square (0.7 x 1.0 µm). No sheath, no constrictions at the septa. Filaments are found extending from the floc structure and free in the bulk solution. May contain intracellular sulfur granules. Usually easy to identify due square sulfur granules as opposed to spherical on other types of filaments under phase contrast. Does not respond to the S test. Poly-ß-hydroxybutric acid (PHB) is frequently observed as dark intracellular granules. May have incidental attached growth. The filament staining is Gram negative or Gram positive when sulfur granules present and Neisser negative with Neisser positive granules observed frequently.
Types 1851 and Type 0803 are similar although Type 0914 occurs in bundles, and neither Types 1851 or Type 0803 have sulfur granules. Thiothrix II is similar also.
This sulfur oxidizing filament is usually found in environments where there are septic wastes and sulfides. It is commonly observed in activated sludge but rarely causes bulking. This filament is usually found in environments where there low F/M (0.02-0.2) with simple sugars and organic acids. N deficiency may also be a cause but very rarely.
F/M can be changed by increased sludge wasting, changing from complete mix to plug flow or use of a selector. Increasing N levels has been shown to reduce bulking. Septic wastes can be altered by pre-aeration or pre-chlorination. Check solids handling in clarifiers, primaries and EQ tanks. Also check for septic supernatant from dewatering processes.
Type 0914 ranks 18th in number of predominance.
For more information on Filamentous Identification
More photos to come. . .
If you need more information on our Filamentous Identification the Easy Way Training CD or on Internet training on Filamentous bacteria, causes and controls.
How and why on Wastewater Biomass Analyses