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Filamentous Identification Lab Service. One reason to identify filaments is to determine the filaments characteristics and then determine the type present. If the type is found out, a root cause can usually be associated with a particular filament. If the cause is known, then a correction can be made to alleviate problems. Chlorination is only a quick fix. Without process changes, filaments will grow back after chlorination.
Wastewater Biomass Analyses and Cooling Tower Analyses also available
Training is an integral part of any job. Not everyone is at the same level of training. Many people want beginning concepts and basics. Some need technical information or troubleshooting. Some want equipment, technology or process information.
We have developed a full set of Basic training, Advanced training, Filamentous Identification the Easy Way as well as custom training CD's Manuals. We also provide hands-on training classes and soon will have an Online "E-University".
We hope you like the new look of our Filamentous Bacteria Identification Pages
If you would like more information on filaments, you might want to consider purchasing our Filamentous Identification the Easy Way Training materials.
We also have our lab that can perform a Filamentous identification lab analysesof your own MLSS for more information
This filament is very common in low D.O. environments. Don't get overly technical. When trying to determine species, stick to the basics, and focus on the causes and controls of the filaments present. The main point of any filamentous identification is not to get a PhD, but to fix your plant!
Relatively short, non-motile filaments (20-100 µm). Bent or curved with no branching. The cells are round-ended and rod shaped “sausages” (0.7-1.0 x 1.0-2.0) are contained in a clear, tightly fitting sheath. The cell septa are clear and easily observable with indentations at the septa. Filaments are usually found intertwined in the floc structures with only short filaments extending into the bulk solution and can cause sludge settling interference by open and diffuse floc. If the filament is observed radiating outward from the floc structure and does not have a lot of attached growth, this is a sign of accelerated growth. The filament is usually Gram negative and Neisser negative. There are no sulfur granules. Poly-ß-hydroxybutric acid (PHB) is almost always observed as dark intracellular granules. In wastewater that is nutrient deficient, an exocellular slime coat may be present. Heavy attached growth is usually common making it difficult to see individual cells. Attached growth is usually perpendicular to the filament trichome.
S. natans may be related, but the diameter is much larger and has distinctive false branching. Type 0675 is similar if false gram staining reaction although Type 0675 has square-ended cells and the cause for Type 0675 is low F/M and not low DO.
This filament is usually found in environments where there is low DO but reasonable F/M ratios. Can be caused by too long RAS lines or sludge being held too long in the secondary clarifier.
RAS chlorination can be used to get rid of the filaments but process changes should also be made. Cell lyses occurs readily on this type of filament, although the empty sheaths still remain. Sludge wasting is necessary to remove them entirely from the system. Manipulation of F/M and DO concentration can be used to control the filaments.
Type 1701 is relatively low in number of predominance. Very rarely causes bulking. Never causes bridging due to the fact the filament is so short.
For more information on Filamentous Identification
More photos to come. . .
If you need more information on our Filamentous Identification the Easy Way Training CD or on Internet training on Filamentous bacteria, causes and controls.
How and why on Wastewater Biomass Analyses